ABSTRACT
OBJECTIVE@#To explore the effect of nitric oxide (NO) on the gene expression of hepatic TNF-α and IL-1β by crush injury of rat's soft tissues.@*METHODS@#Rats were randomly divided into sham group, crush group, crush+aminoguanidine (AG) group, and crush+L-arginine (L-Arg) group. Activities of ALT and AST as well as NO level in serum were measured. Gene expressions of TNF-α and IL-1β were detected with RT-PCR.@*RESULTS@#Obvious increase in TNF-α and IL-1β mRNA expression was detected in the crush group compared with the sham group (P<0.05). After pretreated L-Arg, expressions of TNF-α and IL-1β mRNA were markedly increased (P<0.05). After pretreated AG, those indices obviously decreased (P<0.05). Activities of ALT and AST enhanced and NO level increased in the crush group compared with the sham group (P<0.05). Pretreatment with L-Arg or AG led to substantial increased or reduced activities of ALT and AST as well as NO levels, respectively.@*CONCLUSION@#Endogenous NO mediated TNF-α, IL-1β mRNA up expression in liver induced by increased production of NO after crush injury of rat's soft tissues.
Subject(s)
Animals , Rats , Gene Expression , Interleukin-1beta/metabolism , Liver , Nitric Oxide/physiology , RNA, Messenger , Tumor Necrosis Factor-alpha/metabolism , Wounds and InjuriesABSTRACT
OBJECTIVE@#To explore a new method in order to extract DNA from bones and teeth automatically.@*METHODS@#Samples of 33 bones and 15 teeth were acquired by freeze-mill method and manual method, respectively. DNA materials were extracted and quantified from the triturated samples by AutoMate Express forensic DNA extraction system.@*RESULTS@#DNA extraction from bones and teeth were completed in 3 hours using the AutoMate Express forensic DNA extraction system. There was no statistical difference between the two methods in the DNA concentration of bones. Both bones and teeth got the good STR typing by freeze-mill method, and the DNA concentration of teeth was higher than those by manual method.@*CONCLUSION@#AutoMate Express forensic DNA extraction system is a new method to extract DNA from bones and teeth, which can be applied in forensic practice.
Subject(s)
Humans , Automation , Bone and Bones/chemistry , DNA/isolation & purification , DNA Fingerprinting/methods , Forensic Medicine/methods , Microsatellite Repeats , Polymerase Chain Reaction , Specimen Handling/methods , Tooth/chemistryABSTRACT
<p><b>OBJECTIVE</b>To identify the risk factors of contrast-induced nephropathy (CIN) after coronary interventional therapy (PCI) in patients with coronary heart disease and analyze the clinical outcomes and the preventive measures of CIN.</p><p><b>METHODS</b>Ninety-one patients who developed CIN after PCI were retrospectively analyzed to identify the risk factors and explore the preventive measures.</p><p><b>RESULTS</b>CIN was strongly associated with pre-procedural chronic renal failure, diabetes mellitus and administration of large-dose contrast. The incidence of cardiac mortality in hospital or in the follow-up at one year after PCI, and the incidence of myocardial infarction or major adverse cardiac events in the follow-up at one year were obviously higher in patients with CIN than those without CIN.</p><p><b>CONCLUSION</b>Chronic renal failure, diabetes mellitus and large-dose contrast administration are 3 independent risk factors of CIN, which affects the prognosis of the patients. Reinforcement of a comprehensive perioperative management of PCI, especially a rigorous preoperative preparation, can be an important strategy for prevention of CIN.</p>
Subject(s)
Aged , Female , Humans , Male , Middle Aged , China , Epidemiology , Contrast Media , Coronary Disease , Therapeutics , Kidney Diseases , Epidemiology , Percutaneous Coronary Intervention , Retrospective Studies , Risk FactorsABSTRACT
<p><b>OBJECTIVE</b>To investigate the relation of the levels of C-reactive protein (CRP) and antibodies against oxidized low-density lipoprotein (anti-oxLDL) to with acute coronary syndrome (ACS).</p><p><b>METHODS</b>The levels of CRP, anti-oxLDL and anti-LDL were measured and compared in 96 subjects including 26 with acute myocardial infarction (AMI), 29 with unstable angina pectoris (UAP), 20 with stable angina pectoris (SAP) and 21 control subjects to evaluate the relationship between CRP and anti-oxLDL.</p><p><b>RESULTS</b>Both CRP and anti-oxLDL levels in patients with ACS, including AMI and UAP, were significantly higher than those in SAP patients and the control subjects (P<0.05), but the level of anti-LDL showed no significant difference between the groups (P>0.05). There was significant positive correlation between the levels of CRP and anti-oxLDL (P<0.001).</p><p><b>CONCLUSION</b>The specific immune response to oxLDL may play an important role in the instability of plaque and the occurrence of ACS, and anti-oxLDL level may serve as an important specific marker for the instability of plaque.</p>